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cis-Regulatory control of three cell fate-specific genes in vulval organogenesis of C. elegans and C. briggsae

Citation

Kirouac, Martha (2003) cis-Regulatory control of three cell fate-specific genes in vulval organogenesis of C. elegans and C. briggsae. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-09252002-111831

Abstract

The great-grandprogeny of the Caenorhabditis elegans vulval precursor cells (VPCs) adopt one of the final vulA, B1, B2, C, D, E and F cell types in a precise spatial pattern. Formation of the pattern of vulval cell types is likely to depend upon the cis-regulatory regions of the transcriptional targets of these intercellular signals in vulval development. The outcome of such differential activation will result in individual cell types. egl-17, zmp-1, cdh-3 are expressed differentially in the developing vulva cells, providing a potential readout for different signaling pathways. To understand how different signaling pathways interact to specify unique vulval cell types in a precise pattern, I have identified upstream cis-regulatory regions that are sufficient for their ability to confer vulval cell type-specific regulation when fused in cis to the basal pes-10 promoter. In the egl-17 promoter, I have identified a 143 base pair (bp) region that drives vulC and vulD expression, and a 102 bp region that is sufficient to drive the early expression in presumptive vulE and vulF cells. In the zmp-1 promoter, I have identified a 300 bp region that is sufficient to drive expression in vulE, vulA and the anchor cell. In the cdh-3 promoter, I have identified a 689 bp region sufficient to drive expression in the anchor cell and vulE, vulF, vulD and vulC, a 155 bp region sufficient to drive only anchor cell expression, and a separate 563 bp region that was also sufficient to drive expression in these vulval cells. I have identified the C. briggsae homologs of these three genes, and the corresponding control regions, and tested these regions in both C. elegans and C. briggsae. I find that these regions of similarity in C. elegans and C. briggsae upstream of egl-17, zmp-1, and cdh-3 promote expression in vulval cells and the anchor cell. Using the regions defined by the sufficiency analysis and phylogenetic footprinting, I have been able to isolate over-represented sequences that may play important roles in conferring vulval and anchor cell expression.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:biology; C. elegans; cdh-3; cis-regulation; development; egl-17; genomics; transcription; vulva; zmp-1
Degree Grantor:California Institute of Technology
Division:Biology
Major Option:Biology
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Sternberg, Paul W.
Thesis Committee:
  • Sternberg, Paul W. (chair)
  • Rothenberg, Ellen V.
  • Meyerowitz, Elliot M.
  • Deshaies, Raymond Joseph
  • Fraser, Scott E.
Defense Date:9 September 2002
Author Email:kirouac (AT) caltech.edu
Record Number:CaltechETD:etd-09252002-111831
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-09252002-111831
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3751
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:27 Sep 2002
Last Modified:26 Dec 2012 03:02

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