Best, George C. (1995) Sequence composition and mismatch effects on triple helix formation. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-09062007-134504
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The association constants for the formation of sixteen triple helical complexes which vary at a single common position (Z•XY where Z = A, G, [...], T and XY = AT, GC, CG, TA) were determined by quantitative affinity cleavage titration. The association constants ranged from 9.5 x [...] (A•CG) to 5.6 x [...] (T•AT) at 22 °C, pH 7.0, 10 mM bis-tris, 100 mM NaCl, 250 [...] spermine. For the sequences studied, T•AT is more stable than T•GC, T•CG, or T•TA by [...] 2.3 kcal mol[...]; [...]+GC is more stable than [...]•AT, [...]•CG, or [...]•TA by [...] 1.4 kcal mol[...]; G•TA is more stable than G•AT, G•CG, or G•GC by [...] 0.8 kcal mol[...]. Under the conditions used in these experiments, none of the natural bases form triplets which offer means of recognizing the Watson-Crick CG base pair in a clearly energetically preferred manner.
The association constants for the formation of nine triple helical complexes of varying length and sequence composition were determined by quantitative DNase footprint titration. The association constants ranged from 2.0 x [...] to 3.1 x [...] at 22 °C, pH 7.0, 10 mM bis-tris, 100 mM NaCl, 250 [...]M spermine. The free energy of formation of the various triple helices was expressed as the linear combination of the free energy contributions of the various dinucleotide components of the oligonucleotide, and the value of each energetic contribution was determined. The 5'-TT-3' dinucleotide makes the greatest contribution to the stability of the triple helix, -1.2 kcal•mol[...] dinucleotide[...], while the inclusion of a [...] dinucleotide requires the payment of an energetic penalty of 0.5 kcal•mol[...] dinucleotide[...].
|Item Type:||Thesis (Dissertation (Ph.D.))|
|Degree Grantor:||California Institute of Technology|
|Division:||Chemistry and Chemical Engineering|
|Thesis Availability:||Restricted to Caltech community only|
|Defense Date:||2 September 1994|
|Default Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Imported from ETD-db|
|Deposited On:||11 Sep 2007|
|Last Modified:||26 Dec 2012 02:59|
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