Benbow, Robert Michael (1972) On the genetic recombination of bacteriophage [phi]X174 DNA molecules. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-08082006-132253
Genetic recombination between two [phi]X174 parental replicative form DNA molecules primarily occurs by the following sequence of events: (i) a single strand scission in one HF DNA molecule; (ii) displacement synthesis; (iii) unimolecular branch migration; (iv) attack; (v) formation of a hydrogen bonded joint molecule; (vi) bimolecular branch migration and further DNA (repair?) synthesis; (vii and viii) covalent bond formation and single strand scission (order not certain); (ix) restoration of recombinant DNA molecules to the parental HF configuration. The net result is an asymmetric non-reciprocal recombination event yielding one parent and one recombinant. The average net DNA synthesis is less than 600 nucleotides; breakage and reunion occurs; over 50% of all genetic exchanges involve regions less than 4.00 nucleotides in length. Single strand insertion is (probably) the most common recombination event. Recombinant formation is complete at or shortly after the initiation of progeny HF DNA synthesis; the completed recombinant DNA molecule and surviving parental molecule then resume normal [phi]X174 DNA replication.
A (minor) secondary recombination mechanism exists in which recombinant formation occurs between [phi]X174 progeny HF DNA molecules.
|Item Type:||Thesis (Dissertation (Ph.D.))|
|Degree Grantor:||California Institute of Technology|
|Thesis Availability:||Public (worldwide access)|
|Defense Date:||19 May 1972|
|Default Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Imported from ETD-db|
|Deposited On:||09 Aug 2006|
|Last Modified:||26 Dec 2012 02:56|
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