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Variables of the conformation of the active site of A-chymotrypsin. I. Hydrogen-ion concentration studies. II. Electrolyte concentration studies

Citation

Mukatis, Werner A. (1965) Variables of the conformation of the active site of A-chymotrypsin. I. Hydrogen-ion concentration studies. II. Electrolyte concentration studies. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-01232004-141835

Abstract

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The initial velocities of [alpha]-chymotrypsin-catalyzed hydrolyses of acylated amino acid esters follow the rate law [...] when the only variables are initial substrate and enzyme concentration. In the above equation, [...] is the initial velocity, [...] and [...] are initial enzyme and substrate concentration, respectively, and [...] and [...] are a pair of experimentally determined kinetic parameters. Factors such as temperature, ionic strength, hydrogen-ion concentration, structure of substrate, etc., affect the values of [...] and [...].

Variation in the kinetic parameters [...],[...], and [...] for selected substrates and variations in the ratios of the parameters [...],[...]and [...] for selected pairs of substrates are studied as functions of hydrogen-ion concentration and concentration of added electrolyte.

The results are discussed in terms of possible changes in conformation of the active site of [alpha]-chymotrypsin with changing hydrogenion concentration and concentration of added electrolyte.

Item Type:Thesis (Dissertation (Ph.D.))
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Niemann, Carl G.
Thesis Committee:
  • Unknown, Unknown
Defense Date:3 December 1964
Record Number:CaltechETD:etd-01232004-141835
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-01232004-141835
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:290
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:28 Jan 2004
Last Modified:26 Dec 2012 02:28

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