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I. Genetic studies of mouse serum protein types. II. Molecular hybridization of sheep hemoglobins

Citation

Shreffler, Donald Cecil (1962) I. Genetic studies of mouse serum protein types. II. Molecular hybridization of sheep hemoglobins. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-06292004-095342

Abstract

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Investigations were conducted to detect serum protein variant systems in mice and to define their genetic control. Three systems were detected. A pair of codominant alleles at a locus in the second linkage group controls electrophoretically different transferrin types. An electrophoretic difference among inbred lines in the presence or absence of a prealbumin is controlled by a single autosomal locus without dominance. A serologically detected system is controlled by a single locus closely linked or identical to the H-2 locus in the ninth linkage group. The variant component seems to be an [alpha]-globulin of high molecular weight. The principal difference between serum types is quantitative; qualitative differences are not excluded. The serum component is not detectably related serologically to H-2 erythrocyte antigens. The effects of development, pregnancy and stress upon these serum components were studied. Using these three serum variants as markers, the sera of homologous radiation chimeras were examined for donor type proteins. These occasionally appear transitorily, but long-term survivors having entirely donor erythrocytes have only host type serum proteins.

Molecular hybridizations were performed with the two known sheep hemoglobin variants. Radioactive and ferriheme labels showed some exchange of subunits between variant types, but less than expected. Incomplete exchange is apparently due to inadequate asymmetric dissociation or to partial incompatibilities between both subunits of the two hemoglobins. The electrophoretic difference between the two types resides mainly, if not entirely, in one of the subunits.

Item Type:Thesis (Dissertation (Ph.D.))
Degree Grantor:California Institute of Technology
Division:Biology
Major Option:Biology
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Owen, Ray David
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1962
Record Number:CaltechETD:etd-06292004-095342
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-06292004-095342
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2765
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:29 Jun 2004
Last Modified:26 Dec 2012 02:54

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