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Single mammalian cell gene expression analysis using microfluidics

Citation

Marcus, Joshua Scott (2006) Single mammalian cell gene expression analysis using microfluidics. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-06282006-103230

Abstract

Single cell gene expression studies hold great promise for deciphering the ubiquitous heterogeneity present in biological organisms. Although much progress has been made in the field, tools to study gene expression (specific and global) in single cells are generally lacking. This thesis describes the development of novel microfluidic technologies and processes capable of processing single cells to first strand cDNA in a parallel fashion, thereby filling a void in the single cell biology field. The author then utilizes the technology to probe for transcriptional noise in ubiquitous genes present in single mammalian cells. The noise measured far exceeds any measurement reported to this date, and was shown to be attenuated during the G2 stage of the cell cycle. The work presented here is first hand proof that technological innovation is a key component in undertaking novel science.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:gene expression; microfluidics; noise; qPCR; single cell
Degree Grantor:California Institute of Technology
Division:Biology
Major Option:Biochemistry and Molecular Biophysics
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Quake, Stephen R.
Thesis Committee:
  • Mayo, Stephen L. (chair)
  • Barton, Jacqueline K.
  • Heath, James R.
  • Campbell, Judith L.
  • Quake, Stephen R.
Defense Date:11 April 2006
Author Email:JMarcus (AT) darbylaw.com
Record Number:CaltechETD:etd-06282006-103230
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-06282006-103230
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2755
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:12 Jul 2006
Last Modified:26 Dec 2012 02:54

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