I. Studies on mixed anhydride systems. II. Studies of the alpha chymotrypsin catalyzed hydrolysis of specific substrates

Citation

Mower, Howard F. (1956) I. Studies on mixed anhydride systems. II. Studies of the alpha chymotrypsin catalyzed hydrolysis of specific substrates. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-06162004-132607

Abstract

The synthesis and purification of p-nitrobenzoic-acetic mixed anhydride, p-methoxybenzoic-acetic mixed anhydride and benzoic-acetic mixed anhydride is described. The thermodynamic constants for the disproportionation reaction have been determined. A mechanism for this reaction is discussed. Measurements of the interaction of acetic and benzoic acids with the acetic-benzoic anhydride system have been made.

The cleavage of the mixed anhydrides with nucleophilic agents is described.

The synthesis of the following compounds is described: N-trimethylacetyl-L-tyrosinhydrazide, N-dichloroacetyl-L-tyrosinhydrazide, N-formyl-L-tyrosinhydrazide, N-trichloroacetyl-L-tyrosinamide and N-acetyl-L-cysteic acid carboxylamide. The kinetic constants for the [alpha] chymotrypsin hydrolysis of these substances are reported.

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