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Regulation of neurogenic ectoderm specification in Drosophila melanogaster

Citation

Liberman, Louisa M (2009) Regulation of neurogenic ectoderm specification in Drosophila melanogaster. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-05292009-123115

Abstract

Creating a functional organism requires reproducible developmental patterning. A nuclear gradient of the NF-κB transcription factor, Dorsal, provides positional information necessary to specify the mesoderm, neurogenic ectoderm, dorsal ectoderm and amnioserosa along the dorsal-ventral axis in Drosophila melanogaster embryos. In this work we investigate the role that Dorsal and other transcription factors play in these crucial patterning events. We focus primarily on the gene regulation that controls patterning of the presumptive neurogenic ectoderm that is specified in lateral regions of the embryo. We investigate this early patterning event in two ways: first, by studying a known regulatory element for this region, and second, by examining the levels of Dorsal in the nuclei. We find that Dorsal can function with Zelda, a maternally deposited ubiquitous activator, to specify the neurogenic ectoderm. We then ask if the levels of Dorsal in wild type embryos are predictive of the gene expression outputs, as suggested by existing models. We measure the amount of Dorsal protein able to activate target gene expression in mutants, where the levels of Dorsal protein have been genetically manipulated. Our measurements indicate that Dorsal does not regulate gene expression in a concentration-dependent fashion. Instead, our data support the idea that Dorsal functions with other proteins to establish gene expression boundaries. These studies jointly suggest that regulation of differential gene expression requires combinatorial interactions between spatially localized and uniformly distributed transcription factors.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:cis-regulation; development; Dorsal
Degree Grantor:California Institute of Technology
Division:Biology
Major Option:Biology
Thesis Availability:Restricted to Caltech community only
Research Advisor(s):
  • Stathopoulos, Angelike
Thesis Committee:
  • Sternberg, Paul W. (chair)
  • Stathopoulos, Angelike
  • Davidson, Eric H.
  • Zinn, Kai George
  • Fraser, Scott E.
Defense Date:13 May 2009
Record Number:CaltechETD:etd-05292009-123115
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-05292009-123115
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2273
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:02 Jun 2009
Last Modified:26 Dec 2012 02:49

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