Udell, Edye Carol (1997) Modeling methane utilization by methanotrophs in groundwater : applications for groundwater bioremediation. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-01182008-085109
A model was developed to predict substrate concentrations for bacterial growth in a groundwater system. The model coupled substrate transport and oxidation by cells with bacterial growth. A reactor was designed that could test the model's ability to correctly predict the biological and physical phenomena governing the reactor setup. In order to design the reactor, I needed a methanotroph strain capable of attaching well to sand contained in the reactor. I found that Methylomicrobium albus BG8 cells attached strongly to the pretreated Ottawa sand used for the reactor solid phase. I also analyzed the effect of copper on methane utilization kinetics for this strain because prior evidence suggested copper may influence kinetic parameters. I found that copper did not have a statistically significant effect on methane utilization kinetics under the experimental conditions used. I also found that the mathematical model worked well to predict methane concentrations throughout the reactor over time. Model input parameters were then analyzed for their influence on model predictions. I found that the Michaelis-Menten maximum rate of methane utilization and Monod maximum specific growth rate had the largest effect on model predictions. I also found that mass transfer from the bulk liquid to bacteria attached to particles could have a large impact on model predictions. The mass transfer effect was greatest when the substrate concentration was less than or equal to the Michaelis-Menten affinity coefficient and Monod half-maximum growth rate constant.
|Item Type:||Thesis (Dissertation (Ph.D.))|
|Degree Grantor:||California Institute of Technology|
|Major Option:||Chemical Engineering|
|Thesis Availability:||Restricted to Caltech community only|
|Defense Date:||12 July 1996|
|Default Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Imported from ETD-db|
|Deposited On:||14 Feb 2008|
|Last Modified:||26 Dec 2012 02:28|
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