CaltechTHESIS
  A Caltech Library Service

DNA-templated dimerizations of minor groove–binding polyamides

Citation

Poulin-Kerstien, Adam T. (2005) DNA-templated dimerizations of minor groove–binding polyamides. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-05252005-100011

Abstract

Polyamides have emerged as a class of small molecules capable of binding the minor groove of DNA with high affinity and sequence specificity that have potential applications in molecular biology and human medicine. In efforts towards the use of polyamides in living cells, we report research directed towards DNA-templated formations of polyamide dimers. We find that formation of polyamide dimers, linked both turn-to-turn and turn-to-tail, can be templated via a 1,3-dipolar cycloaddition using a targeted sequence of DNA. The dimer products formed in situ may prove to have interesting biological effects.

Also reported in this thesis are several uses of polyamides as molecular tools. We find that polyamide-biotin conjugates are able to selectively bind and capture targeted pieces of DNA via streptavidin-coated magnetic beads, effectively enriching mixtures of DNA fragments in the fragment of interest. Such molecules may find utility in the identification of DNA-protein complexes. In a second utility we report the use of polyamide-maleimide and chlorambucil conjugates to impart sequence specificity on nonspecific DNA enzymes for crystallographic studies.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:DNA; molecular recognition; polyamide
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Restricted to Caltech community only
Research Advisor(s):
  • Dervan, Peter B.
Thesis Committee:
  • Mayo, Stephen L. (chair)
  • Hsieh-Wilson, Linda C.
  • Dervan, Peter B.
  • Grubbs, Robert H.
Defense Date:17 May 2005
Author Email:kerstien (AT) its.caltech.edu
Record Number:CaltechETD:etd-05252005-100011
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-05252005-100011
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2051
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:26 May 2005
Last Modified:26 Dec 2012 02:46

Thesis Files

[img] PDF (00ATPK2005Thesis.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

20Mb
[img] PDF (01ATPK2005Introduction.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

127Kb
[img] PDF (02ATPK2005Chapter1.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

5Mb
[img] PDF (03ATPK2005Chapter2.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

4Mb
[img] PDF (04ATPK2005Chapter3.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

1768Kb
[img] PDF (05ATPK2005Chapter4.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

2510Kb
[img] PDF (06ATPK2005AppendixI.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

4Mb
[img] PDF (07ATPK2005AppendixII.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

1816Kb
[img] PDF (08ATPK2005AppendixIII.pdf) - Final Version
Restricted to Caltech community only
See Usage Policy.

1133Kb

Repository Staff Only: item control page