In Vitro Selection of mRNA-Display Libraries Containing Unnatural Amino Acids

Citation

Li, Shuwei (2003) In Vitro Selection of mRNA-Display Libraries Containing Unnatural Amino Acids. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/NVCQ-JS95. https://resolver.caltech.edu/CaltechETD:etd-05182003-185245

Abstract

The mRNA display library provides a strategy to make up to 10¹³ unique peptides that fuse with their own genes via a covalent link. In this thesis, several strategies to prepare mRNA display libraries containing unnatural side chains are described. Chapter 1 is a short introduction and application of mRNA-display technology. Chapter 2 describes a chemical derivatization strategy to append an unnatural side chain to libraries. Chapter 2 introduces the incorporation of unnatural amino acids via non-sense codon suppression. Chapter 4 focuses on the catalytic selection with mRNA display libraries. These libraries should facilitate the discovery of novel ligands with functionalities beyond those provided by the 20 naturally occurring amino acids.

Item Type:	Thesis (Dissertation (Ph.D.))
Subject Keywords:	chemical derivatization; mRNA-display libraries; non-sense suppression; unnatural amino acids
Degree Grantor:	California Institute of Technology
Division:	Chemistry and Chemical Engineering
Major Option:	Chemistry
Awards:	The Herbert Newby McCoy Award, 2003.
Thesis Availability:	Public (worldwide access)
Research Advisor(s):	Roberts, Richard W.
Thesis Committee:	Dervan, Peter B. (chair) Bercaw, John E. Roberts, Richard W. Mayo, Stephen L.
Defense Date:	11 March 2003
Record Number:	CaltechETD:etd-05182003-185245
Persistent URL:	https://resolver.caltech.edu/CaltechETD:etd-05182003-185245
DOI:	10.7907/NVCQ-JS95
Default Usage Policy:	No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:	1853
Collection:	CaltechTHESIS
Deposited By:	Imported from ETD-db
Deposited On:	19 May 2003
Last Modified:	19 Feb 2021 00:30