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The fractionation of serum proteins by electrophoresis-convection

Citation

Brown, Raymond A. (1949) The fractionation of serum proteins by electrophoresis-convection. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-05072007-104547

Abstract

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A new improved electrophoresis-convection apparatus for the fractionation of proteins is described. The potentialities of the apparatus are demonstrated in the partial separation of bovine serum proteins and in the sub-fractionation of bovine [...]-globulin, Fraction II obtained by alcohol-salt precipitation. A fraction containing 96% [...]-globulin has been isolated from bovine serum. Bovine [...]-globulin has been separated into eight fractions with mobilities ranging from -1.25x10(-5) to -2.25x10(-5) and isoelectric points extending from pH 7.31 to pH 5.74.

An extension of the theory of reversible boundary spreading in the electrophoresis of proteins is presented. The theory has been applied to the characterization of the [...]-globulin fractions.

Item Type:Thesis (Dissertation (Ph.D.))
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemical Engineering
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Unknown, Unknown
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1949
Record Number:CaltechETD:etd-05072007-104547
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-05072007-104547
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1657
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:14 May 2007
Last Modified:26 Dec 2012 02:40

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