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Preparation and characterization by streaming birefringence of sodium desoxyribonucleate

Citation

Hershman, Jerome Marshall (1953) Preparation and characterization by streaming birefringence of sodium desoxyribonucleate. Master's thesis, California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-05062003-095853

Abstract

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Three preparations of the sodium salt of desoxyribonucleic acid were isolated from calf thymus by a method expected to give minimal or no depolymerization. In addition, preparations were made from calf spleen and bull testis tissues.

Streaming birefringence measurements indicate that the distributions of molecular length are identical in the calf thymus preparations. The apparent molecular lengths range from 7,500 to 11,500[...]. The sodium desoxyribonucleate from testis appeared to be significantly shorter; that from spleen was intermediate between thymus and testis. The distributions of molecular length of two calf thymus preparations isolated by N. Simmons were compared with those prepared here.

Several explanations are put forth to explain the polydispersity and discrepancies between preparations.

Item Type:Thesis (Master's thesis)
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Unknown, Unknown
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1953
Record Number:CaltechETD:etd-05062003-095853
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-05062003-095853
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1643
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:06 May 2003
Last Modified:26 Dec 2012 02:40

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