Bowen, George Hamilton (1953) Kinetic studies on the mechanism of photoreactivation of bacteriophage T2 inactivated by ultraviolet light. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-04212003-134051
Bacteriophage particles are called active if they are capable of generating a plaque when plated on agar by a standard technique. Exposure of the particles to ultraviolet radiation of wave length 253.7 m[mu] inactivates them in this sense. Following adsorption of the inactive particles to sensitive host bacteria, exposure of the suspension to light of the violet and near ultraviolet region causes a fraction of the particles to regain their activity, a phenomenon called photoreactivation.
The kinetics of photoreactivation of bacteriophage T2 have been investigated for the purpose of studying the mechanism by which photoreactivation takes place. The presence of a dark reaction in addition to the light reaction has been demonstrated. The dark reaction precedes the other and has the function of supplying the light-absorbing material which enters into the light reaction. Both the light and the dark reactions follow first-order kinetics.
The amount of photoreactivation produced by a given light treatment is determined by the interaction of the light and dark reactions. This interaction can be described satisfactorily in terms of a simple model for the reaction mechanism.
|Item Type:||Thesis (Dissertation (Ph.D.))|
|Degree Grantor:||California Institute of Technology|
|Thesis Availability:||Public (worldwide access)|
|Defense Date:||1 January 1953|
|Default Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Imported from ETD-db|
|Deposited On:||21 Apr 2003|
|Last Modified:||26 Dec 2012 02:38|
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